Toxicity of fine and coarse atmospheric particles using Vibrio fischeri

In the present study, the toxicity of fine (<2.5m) and coarse (2.5-10um) particulate matter was determined using a rapid and cost-effective bioluminescence assay, the Microtox bioassay. Samples were collected in the city of Kozani which is a heavy industrialized area in the north-western part of Greece and characterized by complex topography. Near Kozani, lignite power stations (PS) operate with a total installed generating capacity of more than 4.7 GW. These PS contribute to about 57% of the total electrical energy produced in Greece. The lignite used by these power stations is mined in the nearby open-pit mines. Dust emissions seem to be the most serious problem in the area, as the measured ambient concentrations of suspended particles are at high levels and exceed local and international standards.

The organic contents from filters were extracted in two stages. Firstly by reflux and then by using ultrasound bath. Then concentration extracts were further concentrated to 1 mL and the half part, after addition of internal standard, was analyzed by gas chromatography - mass spectrometry technique for the determination of the 16 polycyclic aromatic hydrocarbons (PAHs) listed by the U.S. EPA as priority pollutants. The other part of extract was exchanged with 0.5 mL of DMSO and then diluted to freshwater. Following the samples were analyzed for their ecotoxicological properties with Vibrio fischeri. Five consecutive dilutions of the sample were tested in order to determine the EC 50 values (the % of sample concentration that causes 50 % effect on the test organism) as a result of different size of particulates. The results showed correlation between toxicity of airborne particles and PAHs content.



Copyright: © Aristotle University of Thessaloniki
Quelle: 2nd International Conference 2009 (Juni 2009)
Seiten: 5
Preis: € 5,00
Autor: V. Evagelopoulos
Stamatis Zoras
Dr. Petros Samaras
Athanasios G. Triantafyllou
Triantafyllos A.D. Albanis
 
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